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AAT Bioquest

FAQ

Posted on February 9, 2024
What are the different types of enzyme-linked receptors?
Enzyme-Linked Immunosorbent Assay (ELISA)Cell SignalingCellular ProcessesEnzymesPhysiological ProbesReceptor Proteins


Posted on February 9, 2024
What do the different types of enzyme-linked receptors do?
Enzyme-Linked Immunosorbent Assay (ELISA)Cell SignalingCellular ProcessesCellular Structures and OrganellesEnzymesReceptor Proteins


Posted on November 16, 2023
How is the result of a direct ELISA measured?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingEnzymes


Posted on November 16, 2023
How do I optimize a direct ELISA?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein Labeling


Posted on November 16, 2023
What are the common enzymes used as labels for ELISA assays?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)EnzymesHorseradish Peroxidase (HRP) and Poly-HRP


Posted on November 7, 2023
What are the applications of immunochemical techniques?
Antibodies and Proteomics Bioconjugation Chemical Reagents Enzyme-Linked Immunosorbent Assay (ELISA)Cell SignalingCellular ProcessesImmunohistochemistry (IHC)Polymerase Chain Reaction (PCR)Western Blot


Posted on November 7, 2023
What are the most commonly used enzymes for protein detection?
Enzyme-Linked Immunosorbent Assay (ELISA)Biotin and StreptavidinHorseradish Peroxidase (HRP) and Poly-HRPWestern BlotProtein Assays & Analysis


Posted on October 23, 2023
Can COVID-19 induce a cytokine storm?
Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Cell SignalingProtein


Posted on October 23, 2023
What is cytokine release syndrome?
Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Cell SignalingProtein


Posted on October 23, 2023
What cells release cytokines?
Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Cell SignalingProtein


Posted on October 23, 2023
Can cytokines cross the cell membrane?
Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Cell SignalingProtein


Posted on October 23, 2023
Do cytokines affect cell signaling?
Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Cell SignalingProtein


Posted on October 23, 2023
Are interleukins a cytokine?
Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Cell SignalingProtein


Posted on October 23, 2023
How do cytokines affect the immune system?
Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Cell SignalingProtein


Posted on October 23, 2023
Are there any cytokines used for disease treatments?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Cell SignalingProtein


Posted on October 23, 2023
Are cytokines used in immunotherapy?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Cell SignalingProtein


Posted on October 23, 2023
What are the types of cytokines?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Cell SignalingProtein


Posted on October 23, 2023
Which cytokines are involved in inflammation?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Cell SignalingProtein


Posted on August 4, 2023
What are the limitations of direct ELISA?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Cell SignalingCellular Processes


Posted on August 4, 2023
What are the advantages of direct ELISA?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Cell SignalingCellular Processes


Posted on July 21, 2023
What are the steps involved in a colorimetric direct ELISA?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)Cell SignalingCellular Processes


Posted on May 24, 2023
What are the common methods available to detect kinase activities?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA) Förster Resonance Energy Transfer (FRET)EnzymesFluorescenceProteinReal-Time PCR (qPCR)qPCR


Posted on April 18, 2023
What types of antibodies can be labeled using antibody labeling kits?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody LabelingAntibody and Protein LabelingImmunohistochemistry (IHC)Spectral Flow Cytometry


Posted on April 18, 2023
What types of labels can be attached to antibodies?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody LabelingAntibody and Protein LabelingImmunohistochemistry (IHC)Western Blot


Posted on January 30, 2023
What are the types of luminescence?
Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingImmunohistochemistry (IHC)


Posted on October 25, 2022
What are the detection methods for western blot?
Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingImmunohistochemistry (IHC)Secondary AntibodiesWestern Blot


Posted on October 25, 2022
What are the steps involved in western blot?
Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingHorseradish Peroxidase (HRP) and Poly-HRPSecondary AntibodiesWestern Blot


Posted on October 14, 2022
Are coumarins useful for immunofluorescence?
Chemical Reagents Enzyme-Linked Immunosorbent Assay (ELISA)BioconjugationClassic DyesCoumarins


Posted on July 26, 2022
What is Cytokeratin 18?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)Antibody LabelingImmunohistochemistry (IHC)Spectral Flow CytometryWestern Blotflow cytometryimmunocytochemistry


Posted on June 1, 2022
Why is BSA used as a standard?
Antibody and Protein Labeling Enzyme-Linked Immunosorbent Assay (ELISA)Immunohistochemistry (IHC) Antibodies and Proteomics Bioconjugation


Posted on September 29, 2020
What is the difference between Western Blot and ELISA?
Antibodies and Proteomics Chemical Reagents Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingImmunohistochemistry (IHC)Western Blot


Posted on September 17, 2020
How do I set up a Sandwich ELISA?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingELISA


Posted on June 1, 2020
Why streptavidin is better than avidin for binding biotin?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)BiotinBiotin and StreptavidinImmunohistochemistry (IHC)flow cytometryimmunofluorescence


Posted on June 1, 2020
What is the difference between direct and indirect ELISA?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingDirect ELISAPrimary AntibodiesSecondary Antibodies


Posted on June 1, 2020
What is the difference between direct and sandwich ELISA?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingDirect ELISAPrimary Antibodies


Posted on April 24, 2020
What is the difference between Myc and c-Myc tag proteins?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingImmunohistochemistry (IHC)ProteinWestern Blot


Posted on April 24, 2020
Why is TMB used in conjunction with HRP in ELISA?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingHRPHorseradish Peroxidase (HRP) and Poly-HRPImmunohistochemistry (IHC)


Posted on April 24, 2020
Which method is better for measuring the amount of antibodies in a sample, ELISA or FACS?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)ELISAEnzymesPeptide and Oligonucleotide


Posted on April 24, 2020
What is the difference between a serotype and a subtype of a virus?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingDNA and RNAEnzymesProtein


Posted on April 24, 2020
What is the difference between bicarbonate and PBS as a coating buffer in ELISA assay?
Chemical Reagents Enzyme-Linked Immunosorbent Assay (ELISA)Buffer Preparations and RecipesELISAPBSbuffer


Posted on April 24, 2020
What is the difference between capture antibody and marker-antigen?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingELISAIHCImmunohistochemistry (IHC)


Posted on April 17, 2020
What's the role of BSA/Milk as blocking agents??
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingBSABuffer Preparations and RecipesImmunohistochemistry (IHC)bovine serum albuminbuffer


Posted on April 17, 2020
What are some reliable methods for validating antibodies?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingIHCImmunohistochemistry (IHC)Western Blot


Posted on April 17, 2020
What is the role of Tau protein aggregation in neurodegeneration?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingWestern Blot